AFFIRM

Natalizumab

AFFIRM showed large effects of natalizumab monotherapy on relapses, disability progression, and MRI activity in relapsing MS.

Phase
Phase III
Population
Relapsing-remitting multiple sclerosis (RRMS)
Controlled period
Nov 2001 — Nov 2004

What was compared

RandomizedDouble-blindPlacebo-controlledMulticenter
Intervention

Natalizumab

300 mg intravenous infusion every 4 weeks

n=627
Comparator

Placebo

Matching intravenous infusion every 4 weeks

n=315
942 randomized116 weeks controlled phase

Primary endpoint

Prespecified outcome

Clinical relapse and sustained disability progression

Relapse at 1 year; disability at 2 years

Outcome figure

Primary endpoint results

Intervention Comparator or reference

Annualized clinical relapse rate

Relapses per participant-year

1 year
Natalizumab
0.26
Placebo
0.81

12-week sustained disability progression

Participants with progression (%)

2 years
Natalizumab
17%
Placebo
29%

68% lower clinical relapse rate at 1 year and 42% lower risk of sustained disability progression at 2 years.

Selected secondary outcomes

New or enlarging T2 lesions

Mean 1.9 with natalizumab versus 11.0 with placebo over 2 years.

Gadolinium-enhancing lesions

92% fewer with natalizumab at years 1 and 2.

Study population

Randomized
942 randomized
Age
18–50 years eligible
Disability
EDSS 0–5.0
Disease definition
Relapsing-remitting multiple sclerosis with recent relapse

Who entered the trial

Key inclusion criteria

  • Defined based on McDonald criteria 2001
  • At least one relapse in the preceding 12 months
  • MRI lesions consistent with MS and EDSS 0–5.0

Key exclusion criteria

  • Primary progressive, secondary progressive, or progressive-relapsing MS
  • Relapse within 50 days without subsequent stabilization
  • Clinically important infection, immune disorder, or major systemic disease

Safety signal

  • Fatigue and allergic reactions were more frequent with natalizumab.
  • Serious hypersensitivity occurred in 1% of natalizumab-treated participants.
  • The controlled trial was not large or long enough to define rare opportunistic infection risk.

Why it mattered

AFFIRM demonstrated that blocking leukocyte adhesion could produce substantially larger effects than the platform-therapy era had typically shown.

Important limitation

Rare but serious risks cannot be inferred from AFFIRM alone; its efficacy findings must be separated from safety knowledge accumulated outside this monotherapy trial.

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